Determination of cellulase activity and reducing sugar by Dinitrosalicylic ( DNS) acid reagent were optimized. The physiological character of cellulase and the best culture pH, temperature and stability were quested for. 选择了纤维分解细菌酶活测定方法以及DNS法测定还原糖的最适条件,对纤维素分解细菌的生长特性及纤维素酶的最适温度、pH值、温度和pH值的稳定性进行了探索。